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Typical parameters that can be adopted for any HPLC method being developed and evaluated for robustness. Definitely including solution stability- CRM-certified reference materials and over a course of study period- i.e. 7 days solution stability (sample). https://www.longboardscientific.com/blog/2020/9/9/system-suitability-a-check-on-fit-for-purpose monthly 0.5 2020-10-29 https://images.squarespace-cdn.com/content/v1/59b6d65c12abd93aa2c90b91/1603933397992-YNN8LXF506ZFWR1ZJ710/Screen+Shot+2020-10-28+at+7.02.41+PM.png The Beaker Blog - System Suitability- A "check" on fit for purpose. Peak Tailing. which peak here is the “bad actor” - although not super concerning- THCA definitely is “peak tail” vulnerable- why ? The predicted pKa values are all around 2.9 which is approximately equal to the pH of the mobile phase (if 0.1% formic acid modified) so one might expect the acid groups to be about 50% ionized and more polar- although the high organic content of the mobile phase will tend to stabilize the neutral molecule over the ionized form and thus reduce the effective acidity (increase pKa). The observed elution suggests significant additional lipophilicity for the addition of a carboxylic acid group-where most molecules are neutral in this system. https://images.squarespace-cdn.com/content/v1/59b6d65c12abd93aa2c90b91/1603922938015-BQLMZ8L4701R8UVAFY48/Screen+Shot+2020-10-28+at+3.51.58+PM.png The Beaker Blog - System Suitability- A "check" on fit for purpose. 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